Table of Contents
What should you do before switching to high power?
Important Note: Before switching to high power, you should always position the specimen in the center of the field of view and use the fine adjust to sharpen the focus of the image. Never use the coarse adjustment when using high power. Doing so could break the slide or the microscope!
Why should the specimen be placed at the center of the hole of the stage of the microscope?
A fixed platform with an opening in the center allows for the passage of light from an illu-minating source below to the lens system above the stage. This platform provides a surface for the placement of a slide with its specimen over the central opening.
What should you always do before switching to a higher power while using a microscope?
When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power. Therefore, it is best to find an object on low power, center it, and then switch to the next higher power and repeat.
Why it is important to always start to focus a specimen with the stage all the way up?
It will give you the largest field of view. You always start with this lens to initially view the specimens because it gives the largest field of view. With a large field of view, it is easier to find what specific area of the specimen to look for as it gives you a full view of the specimen.
Why the specimen must be centered in the field of view on low power objective before going to high power objective?
The specimen must be centered in the field of view on low power before going to high power because on high power, it is difficult to find the specimen. A microscope has a 20X ocular (eyepiece) and two objectives of 10X and 43X.
What is the important thing to remember as you turn the high power objective into place?
An important thing to remember as you turn the high power objective into place , it is retractable, when you hit a slide, the end of the lens will push in (spring loaded) thereby protecting the lend and the lens.
When you switch to higher magnification What should you do to the light intensity?
The light intensity decreases as magnification increases. There is a fixed amount of light per area, and when you increase the magnification of an area, you look at a smaller area. So you see less light, and the image appears dimmer. Image brightness is inversely proportional to the magnification squared.
Why must proper lighting be obtained when you operate the microscope?
The illumination system of the standard optical microscope is designed to transmit light through a translucent object for viewing. To obtain optimal use of the microscope, it is important that the light from the source both covers the object and fills the entrance aperture of the objective of the microscope with light.
Why is it important to start with the scanning objective in place rather than the high power objective?
Because they stay in focus when you change the objective lense. Why is locating an object more difficult if you start with the high-power objective than with the low-power objective? Because the high power brings the object closer so it might be difficult to focus.
When you focus on a new specimen always begin with?
3. When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective.