Table of Contents
What is the Rf value of Pheophytin?
0.59 to 0.60
The results were in accordance with literature, which states that pheophytin has Rf 0.59 to 0.60, and the spot is grey with the same method9. Spot 3 was identified as chlorophyll b because it was yellowish green with Rf 0.42. Spot 4 was orange with Rf 0.36 and identified as xanthophyll.
What is the Rf value of xanthophyll?
0.15-0.35
Therefore, pigments 1 and 2 are likely to be carotenes, and pigment 4 is likely to be a xanthophyll. Pigment 3 is likely to be chlorophyll, since it is more polar than carotenes but less polar than xanthophylls….Explanation.
| Pigment | Rf value |
|---|---|
| Anthocyanins | 0.32-0.62 |
| Xanthophylls | 0.15-0.35 |
How do you calculate the Rf value of a pigment?
Rf = (distance moved by pigment)/ (distance moved by solvent)
- Rf for carotenes = 9.7cm/9.8cm = 0.99.
- Rf for xanthophylls = 7.2cm/9.8cm = 0.73.
- Rf for chlorophyll a = 5.1cm/9.8cm = 0.52.
- Rf for chlorophyll b = 3.7cm/9.8cm = 0.38.
Why does carotene have a high Rf value?
The Rf value of the xanthophyll is two thirds that of carotene because xanthophyll has an H bond with cellulose, which slows it down, and makes it less soluble in the solvent. Carotene is more soluble in 9:1 petroleum-ether acetone solution, which caused it to be carried higher and have a larger Rf value.
What is the Rf value of lutein?
h. Interpret your results. Expected Rf values: Beta‐Carotene, 0.96; Lutein, 0.247; Violaxanthin, 0.16; Neoxanthin, 0.087.
What does a high Rf value mean?
A high Rf (Ie 0.92) would refer to a substance that is very non-polar. Ie that substance moved a 92% of the entire distance the solvent traveled. A low Rf value (0.10) would refer to a substance that is very polar.
What is Rf value formula?
Formula. Rf = DSU / DSV. Distance Travelled By Solute. Distance Travelled By Solvent.
What are Rf values?
RF value (in chromatography) The distance travelled by a given component divided by the distance travelled by the solvent front. For a given system at a known temperature, it is a characteristic of the component and can be used to identify components.
What does Rf value tell?
The Rf values indicate how soluble the particular pigment is in the solvent by how high the pigment moves on the paper. Two pigments with the same Rf value are likely to be identical molecules. Small Rf values tend to indicate larger, less soluble pigments while the highly soluble pigments have an Rf value near to one.
What are the Rf values of aspirin and Anacin?
Anacin’s Rf values clearly correspond to those of caffeine and aspirin. Substances: Aspirin Caffeine Acetaminophen IbuprofenExcedrin Anacin. Rf Values: 0.45 0.08 0.24 0.60 0.09 0.10 0.26 0.46 0.39. Aspirin Caffeine Ibuprofen Acetaminophen Exc.
How are anthocyanidins used in the food industry?
The profiles of anthocyanins and anthocyanidins present in berry fruits can be used as fingerprints for the evaluation of authenticity of raw materials, juices, fruit extracts, and food products. The aim of our work was to establish the authenticity of fruit juices from the market according to the label inscription.
How does the Rf value of a pigment vary?
The Rf value varies depending on the solvent used, but the general order of the pigments (from the highest to the lowest Rf value) usually remains the same, because the nonpolar compounds move further than the polar compounds. Rf values for various pigments (using hexane, acetone and trichloromethane (3:1:1) for the solvent) are shown in table 1.
How many glycosylated anthocyanidins can you identify?
The RP-HPLC chromatographic fingerprints gave us the possibility to identify seventeen anthocyanins (glycosylated anthocyanidins) and five major anthocyanidins (delphinidin, cyanidin, petunidin, pelargonidin, and malvidin) from the extracts of colored fruits.