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How do you dilute Rhodamine B?

How do you dilute Rhodamine B?

Stock Standard 1 Dissolve 0.01 g of rhodamine B standard in 10 mL of DI water. The concentration of Stock Standard 1 will be 1000 μg/mL. Stock Standard 2 Dilute 200 μL of Stock Standard 1 to 10 mL with DI water.

How do you make a PPM solution for Dye?

1 ppm means 1 mg in 1000 mL. For 500 ppm add 500 mg (0.5 gm) of Rh5 in 1000 mL.

What is meant by 10 ppm?

RSS. Thank You Silver has 10 parts per million (ppm) of silver within our unique pure structured water.

How do you make a 20 ppm solution?

So, if you dissolve 0.05 g of sodium chloride in 1 L of water you’ll get a 20-ppm Na+ solution.

Is Rhodamine B soluble in water?

Rhodamine B

Names
Appearance red to violet powder
Melting point 210 to 211 °C (410 to 412 °F; 483 to 484 K) (Decomposes)
Solubility in water 8 to 15 g/L (20 °C)
Hazards

Is Rhodamine B cationic or anionic?

In addition, other cationic dyes, like malachite green (MG) and rhodamine B (RhB), as well as anionic dyes, like indigo carmine (IC), acid fuchsine (AF), acid orange (AO), and fluorescein disodium salt (FD), are also tested in this work.

What is ppm 12 chemistry?

Class 12 Chemistry Solutions. Parts per million. Parts per million. Parts per million can be defined as the ratio of number of parts of the component to the total number of parts of all components of the solution multiplied by 106. It is denoted by ppm.

How do you prepare a liquid ppm solution?

1000ppm =1000mg/L. It’s equivalent to 1g/L or 0.1g/100ml. It’s a measure of mass per volume. To achieve this it’s advisable to use standard volume which dilution factor can be achieved with ease, e. g. 10000ml = 10L, 1000ml = 1L, 100ml, 10ml.

How do you make a dye solution?

To make a 1% solution, weigh 10g of dye and combine with 1000ml of water. Create 0.1% dye stocks by measuring 1 gram of dye powder and mixing with 1000 ml of water, or by mixing 100ml of a 1% solution with 900ml of water (either way, the ratio of dye powder to volume of water is the same).

What is the molecular weight of methylene blue?

319.85 g/mol
Methylene blue/Molar mass

Methylene blue dye (MBD) (CI No. 52015) is a heterocyclic aromatic chemical compound with the molecular formula of C16H18N3SCl and molecular weight of 319.85 g/mol.

How much Rhodamine WT is in one liter?

Note: Rhodamine WT comes as a 20% solution in water(meaning it is 20% active ingredient). The EPA guidelines are interms of active ingredient. Thus, one microgram of the 20%solution in one liter is 0.2 ppb active ingredient (or 20% of 1ppb).

Is the reagent Lissamine Rhodamine B soluble in DMF?

Lissamine rhodamine B sulfonyl hydrazine is soluble in DMF. The reagent may be dissolved in this solvent as a concentrated stock solution before adding a small aliquot to an aqueous reaction medium. The compound itself and all solutions made with it should be protected from light to avoid decomposition of its fluorescent properties.

How does sulfonyl hydrazine react with Lissamine Rhodamine B?

Lissamine rhodamine B sulfonyl hydrazine reacts with aldehyde groups to form hydrazone linkages. Lissamine rhodamine B sulfonyl hydrazine is soluble in DMF. The reagent may be dissolved in this solvent as a concentrated stock solution before adding a small aliquot to an aqueous reaction medium.