What is deuterium lamp used for?

What is deuterium lamp used for?

A deuterium arc lamp (or simply deuterium lamp) is a low-pressure gas-discharge light source often used in spectroscopy when a continuous spectrum in the ultraviolet region is needed.

Which lamp is used in UV spectrophotometer?

tungsten lamp
In UV spectrophotometer, the lamp used for UV absorption measurement is tungsten lamp.

Why does a deuterium lamp produce a continuum rather than a line spectrum in the UV?

Why does a deuterium lamp produce a continuum rather than a line spectrum? In a deuterium lamp, the lamp energy from the power source produces an excited deuterium molecule that dissociates into two atoms in the ground state and a photon of radiation. Consequently, the emission spectrum is a spectral continuum.

Why deuterium lamp is used in HPLC?

Deuterium lamps emit an almost continuous spectrum of light ranging from the main UV wavelengths of 160 – 400 nm to the visible spectral range (800 nm). This makes them the ideal light source for high precision absorption measurements, e.g. in HPLC.

Which lamp is used in visible region?

Fluorescent lamps are about two to four times more efficient at emitting visible light, produce less waste heat, and typically last ten to twenty times longer than incandescent lamps.

What is the principle of UV?

Principle of UV Spectroscopy As light is absorbed by matter, the result is an increase in the energy content of the atoms or molecules. When ultraviolet radiations are absorbed, this results in the excitation of the electrons from the ground state towards a higher energy state.

Why quartz cuvette is used in UV?

Historically, reusable quartz cuvettes were required for measurements in the ultraviolet range, because glass and most plastics absorb ultraviolet light, creating interference. Glass, plastic and quartz cuvettes are all suitable for measurements made at longer wavelengths, such as in the visible light range.

Why are Phototubes not suitable for IR instruments?

A caution when using a photomultiplier tube is that it must not be exposed to too high an intensity of radiation, since high intensity radiation can damage the photoelectric surface. It is not useful for measuring the intensity of low energy radiation in the infrared and microwave portion of the spectrum.

When comparing filters and monochromators as wavelength selectors which one is better suited for qualitative analysis or structural studies?

Filters and monochromators as wavelength selectors. Filters provide low resolution wavelength selection often suitable for quantitative work, but not for qualitative analysis or structural studies. Monochromators produce high resolution (narrow bandwidths) for both qualitative and quantitative work.

Why we use UV detector in HPLC?

HPLC UV detectors are used with high performance liquid chromatography to detect and identify analytes in the sample. By measuring the sample’s absorption of light at different wavelengths, the analyte can be identified.

When should deuterium lamps be replaced?

When to replace a lamp If the peaks of interest have a strong signal, in other words, in excess of 100 mAU/mV, then it is reasonable to wait until the lamp fails to ignite until it is replaced. This will typically be 4,000–8,000 hours.

Why UV visible spectroscopy is used?

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and certain biological macromolecules. Measurement is usually carried out in solution.

Why is deuterium used instead of hydrogen in a lamp?

Deuterium is used rather than hydrogen because of its greater intensity of UV emission in the molecular band. A deuterium lamp uses a tungsten filament and anode placed on opposite sides of a nickel box structure designed to produce the best output spectrum. Unlike an incandescent bulb, the filament is not the source of light in deuterium lamps.

How much radiation does a deuterium arc lamp emit?

The deuterium lamp emits radiation extending from 112 nm to 900 nm, although its continuous spectrum is only from 180 nm to 370 nm. The spectrum intensity does not actually decrease from 250 nm to 300 nm as shown in the spectrum plot above.

How does the emission spectrum of deuterium differ from protium?

The emission spectrum of deuterium differs slightly from that of protium due to the influence of hyperfine interactions, though these effects alter the wavelength of the lines by mere fractions of a nanometer and are too fine to be discerned by the spectrometer used here.

What kind of radiation does a UV lamp produce?

UV lamps produce light radiation from ultraviolet through visible to infrared radiation. The biggest hazard comes from the UVR. UVR cannot be seen and is not felt immediately, but it is harmful to both the eyes and skin. The user may not realize the danger until after the exposure has caused damage. Symptoms can occur 4 to 24 hours after exposure.