What is the advantage of a Gram stain over a simple stain?

What is the advantage of a Gram stain over a simple stain?

What is the advantage of the Gram stain over a simple stain such as methylene blue? Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria.

What is the major advantage of a differential stain such as the Gram stain over a simple stain quizlet?

The advantage of a differential stain over a simple stain is the differential stain allows you to observe cell morphology and structural components of the cell, while the simple stain only allows you to observe cell shape, size and arrangement.

What is the current theory about the mechanism of Gram stain reaction?

With the current theory behind Gram staining, it is thought that in Gram-positive bacteria, the crystal violet and iodine combine to form a larger molecule that precipitates out within the cell.

What is the major advantage of a differential stain such as the Gram stain over a simple stain was this observed in your experiment?

What are the advantages of differential staining procedures over the simple staining technique? Differential staining allows one to differentiate G+ from G- cells, whereas simple staining only shows cell size and morphology.

Are Saprophytic mycobacteria acid-fast?

Saprophytic Mycobacteria are acid-fast positive and don’t cause disease. The kinds of acid-fast organisms that do cause disease, like Mycobacterium tuberculosis, are more difficult to treat with antibiotics because of the large amounts of lipids in their cell walls so they do cause serious disease.

What Colour is Gram positive?

The staining method uses crystal violet dye, which is retained by the thick peptidoglycan cell wall found in gram-positive organisms. This reaction gives gram-positive organisms a blue color when viewed under a microscope.

What is the difference between simple stain and Gram stain?

The Gram stain is a differential stain, as opposed to the simple stain which uses 1 dye. As a result of the use of 2 dyes, making this procedure a differential stain, bacteria will either become purple/blue or pink during the procedure.

Are Saprophytic mycobacteria acid fast?

What is the basis of gram staining?

The basic principle of gram staining involves the ability of the bacterial cell wall to retain the crystal violet dye during solvent treatment. Gram-positive microorganisms have higher peptidoglycan content, whereas gram-negative organisms have higher lipid content.

What is the most crucial step in Gram staining?

The critical step of the Gram staining procedure is the decolorization step. Hold the slide in a tilted downward position and allow the decolorizer to flow over the smear. Be careful not to miss any portion of the smear. Usually a few seconds will suffice.

What is the size of staphylococci in micrometers in centimeters quizlet?

The size of staphylococci in micrometers is 0.5 to 1 micrometer. In centimeters is 0.00005 to 0.0001 cm.

How big is the spherical cell of Staphylococcus aureus?

description In staphylococcus …various strains of the species S aureus and S. epidermidis. In bacteria: Diversity of structure of bacteria …and the spherical cells of Staphylococcus aureus are up to 1 μm in diameter.

How does Staphylococcus aureus affect the body?

osteomyelitis In osteomyelitis …caused by the infectious organism Staphylococcus aureus, which reaches the bone via the bloodstream or by extension from a local injury; inflammation follows with destruction of the cancellous (porous) bone and marrow, loss of blood supply, and bone death. Living bone grows around the infected area and walls in the….

How many colonies of Staphylococcus aureus can you put on a plate?

Select plates containing 20-200 colonies, unless only plates at lower dilutions (>200 colonies) have colonies with typical appearance of S. aureus.

How long to incubate agar for Staphylococcus aureus?

Make certain inoculum reaches bottom of tube. Cover surface of agar with layer of sterile paraffin oil at least 25 mm thick. Incubate 5 days at 35-37°C. Acid is produced anaerobically if indicator changes to yellow throughout tube, indicating presence of S. aureus.