Why do you need to be careful when focusing a microscope?
WHEN YOU ARE DOING THIS FOCUSING, IT IS IMPORTANT TO BE VERY CAREFUL. IT WILL NOT TAKE MUCH ROTATION OF THE FINE FOCUS KNOB TO CAUSE THE 100X OBJECTIVE TO RAM THROUGH THE SLIDE, DAMAGING BOTH THE OBJECTIVE AND THE SLIDE.
What are common errors in using a microscope?
The three most common errors in the use of the operating microscope are using magnification that is too high, changing techniques too rapidly, and a lack of practice.
What mistakes should you avoid when using a microscope?
How to avoid the most common mistakes when using your microscope
- Poor slide preparation. A smudged slide.
- Insufficient magnification. Insufficient magnification.
- Stay focussed. The last common issue is the out-of-focus image.
- Centre the lifeforms in the middle of your slide.
What should you do if you Cannot find the specimen you are observing when you look through the microscope?
If you cannot see anything, move the slide slightly while viewing and focusing. If nothing appears, reduce the light and repeat step 4. Once in focus on low power, center the object of interest by moving the slide. Rotate the objective to the medium power and adjust the fine focus only.
Why the microscope Cannot be used anytime?
Since the microscope uses visible light and visible light has a set range of wavelengths. The microscope can’t produce the image of an object that is smaller than the length of the light wave. Any object that’s less than half the wavelength of the microscope’s illumination source is not visible under that microscope.
What could go wrong when making a microscope slide?
Most problems with air bubbles come from not applying the coverslip at an angle, not touching the liquid drop, or from using a viscous (thick) liquid. If the liquid drop is too large, the coverslip will float on the slide, making it hard to focus on the subject using a microscope.
What should you be careful of when using a microscope sample?
Microscope Safety
- Clean the microscope after each use.
- Handle glass slides carefully.
- Turn off the light source when the microscope is not in use.
- Be aware if your microscope has a mercury lamp.
- When carrying the microscope, always use two hands with one hand supporting the base and theother hand holding the arm.