Why is saline used in bacteria serial dilution?

Why is saline used in bacteria serial dilution?

Saline water isotonic solution (no swelling or shrinking of cell) same as cell protoplasm, minimizes the chances of cell death. Serial dilution or culture suspension used saline solution (0.9% Nacl) because 0.9% sodium chloride (Nacl) solution is isotonic in nature.

What was the purpose of the saline?

Saline has many uses in medicine. It’s used to clean wounds, clear sinuses, and treat dehydration. It can be applied topically or used intravenously.

Why is saline used in microbiology?

Due to the isotonic nature of normal saline that is why its is used in making bacterial suspension, because the cells get acclimatized and escape been ruptured by becoming turgid when the solution used is hypersonic; or plasmolysis resulting from suspending the cells in a hypotonic solution.

What diluent is used in serial dilution?

Serial dilution involves the process of taking a sample and diluting it through a series of standard volumes of sterile diluent, which can either be distilled water or 0.9 % saline.

Can bacteria grow in saline solution?

To determine the capacity of organisms for growing in saline as compared with dextrose solution and tri-destilled sterile water. Conclusions: The 0.9% saline solution can support significative growing of potentially pathogenic bacteria.

How do you make saline solution for serial dilutions?

Saline Solution, 0.9% Sodium Chloride (NaCl)

1. Dissolve 9 g NaCl (mw 58.44) in 700 ml deionized or distilled water in clean container.
2. Add water to bring total solution volume to 1000 ml.
3. Make 10 ml aliquots in sterile 15 ml culture tubes.

Why is saline used instead of water?

Saline solution is specially formulated to match the electrolytes present in blood plasma, therefore, causing less of an osmotic effect compared to other intravenous fluids. It contains sodium and chloride ions as electrolytes. Also, saline water is used in various procedures, such as hemodialysis.

How does a saline solution work?

The saline solution rinses out your nasal passages. The saltwater also restores moisture and eases inflammation of the mucous membranes that line your sinuses. Tiny hair-like “cilia” in those membranes pass bacteria and other junk to the throat, where you harmlessly swallow them.

How do you make saline water for serial dilutions?

Can bacteria grow in saline water?

Material and methods: Experimental controlled study. Different microbial strains were innoculated in aqueous solutions (5% dextrose and 0.9% saline) as well as tri-destilled sterile water. Conclusions: The 0.9% saline solution can support significative growing of potentially pathogenic bacteria.

What is the purpose of serial dilution chemistry?

Serial dilutions are used to accurately create extremely diluted solutions, as well as solutions for experiments that require a concentration curve with an exponential or logarithmic scale. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics.

Why are serial dilutions more accurate?

The more evenly spaced the calibration standards are over this range, makes the results of the analysis more reliable. Each successive standard uses a small portion of the previous standard, which is diluted by solvent to generate the next calibration standard in the series.

Which is the best saline solution for serial dilution?

Phosphate buffer saline (PBS) solution also suitable for serial dilution preparation. Buffered sodium chloride peptone (BSCP) solution or peptone solution is not suitable for serial dilution because it supports growth and after utilizing the nutrients (peptone) bacterial cell die. Saline is 0.9% sodium chloride (Nacl) solution.

Why is saline used in the microbiology field?

Learn the fundamentals of business and how to apply them in your healthcare practice. “Saline solution is used for the preparation of culture suspension or serial dilution in microbiology. Saline is 0.9 % sodium chloride solution. Culture suspensions are prepared in saline solution because 0.9 % sodium chloride solution is isotonic in nature.

What’s the difference between sterile saline and saline solution?

A distinction should further be made between sterile water and saline solution. Sterile Saline solution has 0.9% Sodium Chloride and is used for wound irrigation and cleaning. What would happen if pure water was used as IV fluid instead of saline solution?

Why is 0.9% saline solution used in culture suspension?

Serial dilution or culture suspension used saline solution (0.9% Nacl) because 0.9% sodium chloride (Nacl) solution is isotonic in nature. In isotonic solution the concentration of solutes remains the same both inside and outside of the microbial cell and cells remains at their usual osmotic pressure.