How is nutrient broth sterilized?
Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. The prepared medium should be stored at 2-8°C.
What is a sterile broth?
In order to protect sterile broth, plates, slants and pure cultures from the microbes all around us, we must practice sterile (aseptic) technique. This simply means that sterile surfaces or sterile media must be protected from contamination by microbes in the air or residing on non-sterile surfaces.
What is the purpose of media sterilization?
Media sterilization is the destruction or removal of all forms of microbial life from the aqueous feedstock. In industrial fermentations, components such as vessels, pipework, media, inlet air, and exhaust gases are frequently sterilized by a combination of wet-heat and filtration methods.
Why should petri dish not be left open?
Petri dishes should not be kept open to reduce the risk of contamination by air microbes. Cross contamination can occur as a result. If a petri plate becomes contaminated by microbes, it will interfere with the growth of the target microbes within the plates, and it will hamper the cell culture process.
What is the purpose of broth in microbiology?
Broth cultures are a method of growing bacteria in a liquid growth medium. They’re used to grow and maintain cultures for a laboratory. Different bacteria may grow differently in broth cultures.
Why is nutrient broth necessary?
Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients.
Why is agar sterilized before use?
Preparing the agar plates for growth of a colony of bacteria Glass petri dishes and agar gel must be sterilised before use by using an autoclave , or pre-sterilised plastic petri dishes can be bought. Reason – this will kill any unwanted bacteria that are present in the solution or on the petri dishes.
What is the difference between agar and broth?
The only difference between broth and agar media is that broths do not contain an agar component. We use broth tubes primarily for specific assays, or (rarely) for bacteria that will not form colonies on a solid surface. Unlike preparation of agar plates, tubes are prepared with media already in the incubation vessel.