What is the advantage and disadvantage of pour plate method?

What is the advantage and disadvantage of pour plate method?

Disadvantages of Pour plate method Preparation for the pour plate method is time-consuming compared with the streak plate/and or spread plate technique. Loss of viability of heat-sensitive organisms coming into contact with hot agar. Embedded colonies are much smaller than those which happen to be on the surface.

Why is pour plate better than spread plate?

Generally, pour plates is the method for counting the number of colony-forming bacteria present in a liquid specimen. Pour plates also allow the identification of bacteria as aerobes, anaerobes or facultative aerobes. On the other hand, spread plates allow the isolation of specific clonal colonies.

What is a pour plate?

: a plate prepared by mixing the inoculum with the cooled but still fluid medium before pouring the latter into the petri dish.

Which method is better spread plate or pour plate?

With regard to the accuracy of these two techniques, pour plate has a higher accuracy than the spread plate. Moreover, unlike in a pour plate, a glass spreader is used to spread the sample evenly on the surface on a spread plate.

What are advantages of the streak plate method when compared to the pour plate method?

What advantage does the streak plate method have over the pour-plate method? The streak plate method does not require any additional media for dilution and only requires one plate for inoculation.

Which of the following would be a disadvantage to using the pour plate technique?

What is a disadvantage of the pour plate technique? Sometimes bacteria will clump together and look like a colony when they are not.

What advantages does the pour plate method have over the streak plate method?

What advantage does the pour-plate method have over the streak plate method? The pour plate method requires less skill, has optimization built in, and will more likely produce the desired result. Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation?

What are the advantages of streak plate method compared to pour plate method?

Advantages. Moreover, streak plate is important for the isolation of bacterial cultures by preparing distinct colonies while the pour plate is important for the quantification of colonies in a solid medium.

What precautions should be taken when the pour plates?

11 Pour Plate Method Best Practices

• Keep the molten agar in the water bath for no more than three to four hours.
• Don’t re-melt the agar.
• Use phosphate buffer pH 7.2 if necessary to dilute the suspension.
• Decrease the risk of contamination by pouring plates in a laminar-airflow cabinet.

Which plating method is best?

The spread plate method is better and commonly used having better safety especially during cutting and sealing of wells and also the scope of use of plates for as many types of bacteria as you wish (planning can be at any stage) but in pour plate method the restrictions are created, can be used for the specific …

Which are the advantages of the quadrant streak plate method when compared to the pour plate method quizlet?

What is the advantage of using the streak plate method over the pour plate method? It is quick and takes less materials. You have a nutrient broth, an agar slant, and an agar plate with bacterial growth in or on each.

Which are advantages of the quadrant streak plate method when compared to the pour plate method chegg?

It requires less skill. It is a very quick process. It does not require the use of heat.

What are the advantages of the pour plate method?

Advantages of Pour Plate Technique Easy to undertake. Will detect lower concentrations than surface spread method because of the larger sample volume. Requires no predrying of the agar surface. The most common method for determining the total viable count is the pour-plate method.

Why are pour plates used in colony counting?

Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar medium, a larger volume can be used than with the spread plat e.

Which is easier to detect surface spread or pour plate?

Easy to undertake. Will detect lower concentrations than surface spread method because of the larger sample volume. It requires no pre-drying of the agar surface. The most common method for determining the total viable count is the pour-plate method. The pour plate technique can be used to determine the number of microbes/ mL in a specimen.

Which is better pour plate or streak plate?

The pour plate method requires somewhat less skill than that required for a good streak plate; a disadvantage, however, is that it requires more media, tubes, and plates. Read More