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Why are Vero cells used for viruses?
Firstly, the cell bank is easy to establish and preserve, at the same time it can be continuously passaged with a fast growth rate. Secondly, Vero cells have stable genetic traits and a low probability of malignancy. Thirdly, Vero cells are sensitive to a variety of viruses and have high virus titers.
What are African green monkey kidney cell lines used for?
Preliminary studies indicated that an African green monkey kidney cell line (Vero) is a suitable system for the primary isolation and cultivation of influenza A viruses (E. A. Govorkova, N. V.
What is monkey kidney culture?
Vero cells are a lineage of cells used in cell cultures. The ‘Vero’ lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species).
What is the virus inoculation by cell culture?
The supernatant obtained is used in cell culture. In this method, fungi, cells, bacteria, and blood remain at the bottom of tube (pellet form), whereas viruses remain dispersed in the liquid. Then, 0.2–0.3 mL of the liquid is added to the cell culture medium for absorption of the virus (inoculation).
When did scientists know that monkeys had viruses?
By 1960, scientists and vaccine manufacturers knew that monkey kidneys were sewers of simian viruses. Such contamination often spoiled cultures, including those of an NIH researcher named Bernice Eddy, who worked on vaccine safety.
What can you do with African green monkey kidney cells?
Preliminary studies indicated that an African green monkey kidney cell line (Vero) is a suitable system for the primary isolation and cultivation of influenza A viruses (E. A. Govorkova, N. V. Kaverin, L. V. Gubareva, B. Meignier, and R. G. Webster, J. Infect. Dis.
Which is host cell system does African green monkey use?
African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. E A Govorkova, G Murti, B Meignier, C de Taisne, and R G Webster Department of Virology & Molecular Biology, St. Jude Children’s Research Hospital, Memphis, Tennessee 38105, USA. Author information ►Copyright and License information ►
Can a virus grow only in human cells?
The Chinese have grown a sample but have not yet shared it with the international community. But the Doherty had been preparing for this for years. Many viruses are fussy, and will grow only in a certain type of cell. The lab’s freezers house a huge collection of cells from humans and animals.