Table of Contents
What is the purpose of an agar slant?
Agar slants are commonly used to generate stocks of bacteria. Agar plates can be used to separate mixtures of bacteria and to observe colony characteristics of different species of bacteria (you will perform an experiment in this lab to illustrate this).
What is the main use of a slant in the microbiology lab?
Different Types Of Media For Bacterial Growth: SLANT: solid medium made with agar and various nutrients and indicators. Slanting gives the bacteria a greater surface area on which to grow in a tube. Agar slants are also useful in maintaining bacterial cultures, more so than stacks of Petri dishes.
What is the purpose of using agar plate agar slant and broth in culture for microorganisms?
Agar plates will be used for isolation and some assays, and for short term maintenance of cultures. Agar slant tubes will be used for long term maintenance of isolates. Broths (liquid media) will be used to grow isolates for some assays or for the assays themselves.
Why are agar slants better?
Why are agar slants better suited than agar plates to maintain stock cultures? Slants are better suited because they can be capped, preventing the agar and culture from drying out. The cap also prevents airborne contaminates from entering the slant. Slants take up less storage space.
What is the use of an agar slant and agar deep?
What are agar deep used for? Agar deeps are used to grow bacteria that require LESS oxygen then is present on the surface of the medium. They also aid in determining oxygen requirements and motility of bacteria. Motile bacteria will grow/ move away from the point of inoculation.
What is the primary use of slants and why?
Micro Lab Exercise 4
| Question | Answer |
|---|---|
| What is the primary use of slants? | Long-term storage. Pure cultures. |
| What is the primary use of deeps? | To store anaerobic bacteria. To test oxygen requirements. |
| What is the primary use of broths? | To grow large amounts of bacteria. |
What is an advantage of an agar plate over a slant tube?
What are some advantages of an agar plate over a slant tube? Agar slants are useful in maintaining growth of microorganism over long periods of time without allowing the cultures to dry out. The agar plate is beneficial over a slant tube because it allows for observation, easy access, and isolation of various cultures.
What is the purpose of using the following preparations agar Deeps slants deep slants?
What is the difference between agar slants and deep agar where these are used?
If the medium in the test tube is allowed to harden in a slanted position, the tube is designated an agar slant; if the tube is allowed to harden in an upright position, the tube is designated an agar deep tube; and if the agar is poured into a Petri dish, the plate is designated an agar plate.
What is the purpose of agar slant in microbiology?
Agar slants are useful in maintaining growth of microorganism over long periods of time without allowing the cultures to dry out. The agar plate is beneficial over a slant tube because it allows for observation, easy access, and isolation of various cultures.
What is the function of an agar deep?
Agar deeps are used to grow bacteria that require LESS oxygen then is present on the surface of the medium. They also aid in determining oxygen requirements and motility of bacteria.
What is agar stab?
An Agar Deep Stab is a tube filled with a solid medium containing agar. It is inoculated with a needle to observe the growth patterns in the agar.
What is slant in microbiology?
slant culture. [′slant ‚kəl·chər] (microbiology) A method for maintaining bacteria in which the inoculum is streaked on the surface of agar that has solidified in inclined glass tubes.