Table of Contents
How do you make bromophenol solution?
Dissolve 5.0 g of bromophenol blue powder (tetrabromophenolsulfonphthalein) in 74.5 mL of 0.1 N sodium hydroxide (NaOH) solution. Dilute with purified water to 500 mL. Color and pH range: yellow 3.0-4.6 blue.
How is bromophenol blue prepared for gel electrophoresis?
Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB, albutest) is used as a pH indicator, an electrophoretic color marker, and a dye. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.
How do you use bromophenol blue?
Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain. In gel electrophoresis Bromophenol Blue can be used as a color marker and as as a biological stain. Bromophenol Blue can be used to stain proteins and nucleic acids.
How do you make 6x bromophenol blue?
1) Add 25 mg of bromophenol blue to 6.7 ml of ddH2O and mix. 2) Add 25 mg of xylene cyanol FF and mix. 3) Add 3.3 ml of glycerol and mix. 4) Aliquot and freeze at -20 °C for long-term storage.
What is the concentration of bromophenol blue?
From the first method, the concentration for the Tube A bromophenol blue is 3.15 mg/L while the concentration of Tube B bromophenol blue is 2.05 mg/L.
How do you make 1X loading dye?
Dilute one part 6X Dye solution into five parts of sample solution to give a final concentration of 1X Dye solution. The sample is then ready to load to a gel. For Example: 10µl sample and 2µl 6X Dye Solution. Mix equal volumes of 2X Dye Solution and RNA sample solution to give a final concentration of 1X Dye solution.
Why is my bromophenol blue yellow?
Bromophenol Blue is a pH indicator dye that turns yellow under acidic conditions. Bromophenol Blue has a pI below pH 4.0. The concentrated dye solution may have a reddish or greenish cast to the color. If the pH gradient of the gel includes the pI of the dye, it will turn yellow at its pI.