Table of Contents
What could be a disadvantage of the pour plate method?
Disadvantages of Pour plate method Preparation for the pour plate method is time-consuming compared with the streak plate/and or spread plate technique. Loss of viability of heat-sensitive organisms coming into contact with hot agar. Embedded colonies are much smaller than those which happen to be on the surface.
What is the difference between streak method and pour plate method?
Streak plate refers to a rapid qualitative isolation method for obtaining discrete colonies from a mixed population while pour plate refers to the method of choice for counting the number of colony-forming bacteria present in a liquid specimen.
What advantage does the pour plate method have over the streak plate method?
What advantage does the pour-plate method have over the streak plate method? The pour plate method requires less skill, has optimization built in, and will more likely produce the desired result. Why is the loop flamed before it is placed in a culture tube?
What factors could account for an absence of growth on a streak plate?
Streak Plate – should appear near or in a vaccinity of streak lines and sections. E9 – What factor(s) could accunt for an absence of growth on a pour plate? The lack of oxygen, poor/ bad bacteria smear, or difference in temperature that prevents optimal growth, within media.
Would anaerobic bacteria grow in a pour plate?
Viable anaerobic bacteria are quantitated by the fractional gram pour plate technique under an anaerobic atmosphere. Caution must be exercised when applying the method since isolates may be pathogenic.
What’s the purpose of a streak plate?
Agar streak plates are an essential tool in microbiology. They allow bacteria and fungi to grow on a semi-solid surface to produce discrete colonies. These colonies can be used to help identify the organism, purify the strain free of contaminants, and produce a pure genetic clone.
Why is the streak plate preferred over?
why is streak plate preferred over spot inoculation in Blood Agar procedure? Quadrant streak is better for colony isolation as you start out with a much denser same and then isolate it down, making the test more reliable.
Why is bacteria not growing in petri dish?
Your bacteria may not be growing because if wasn’t placed in a warm or enriched environment. Hope this helps!
Why can some bacteria not grow on any known culture media at all?
Some of the possible reasons are that a required nutrient is not present in the culture medium, that the culture medium itself is toxic, or that other bacteria in the sample produce substances inhibitory to the target organism. Of course, a bacterium dependent on another will not be able to grow independently in vitro.
Can bacteria grow on a streak plate?
Can some bacteria grow on the streak plate and not be seen if the pour plate technique is used? Yes, the pour plate is O2 limited. Thus, some bacteria will only grow on the streak plate as it provides ample O2.
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