Table of Contents
What is arithmetic method of Reed and Muench?
The Reed–Muench method is a simple method for determining 50% endpoints in experimental biology, that is, the concentration of a test substance that produces an effect of interest in half of the test units.
How do you calculate TCID50?
The titer of the viral stock is calculated as TCID50 /ml = 20 x dilution at which CPE develops in only ½ of replicate cultures. The multiplication factor of 20 reflects the fact that only 1/20th of a ml is added to each culture of 293 cells.
What is the Spearman Karber method?
Spearman-Karber method x0 = log10 of the reciprocal of the highest dilution (lowest concentration) at which all animals are positive; ni = number of animals used in each individual dilution (after discounting accidental deaths); ri = number of positive animals (out of ni). Summation is started at dilution x0.
What is proportionate distance?
If an object travels at a constant speed, then the distance traveled is directly proportional to the time spent traveling, with the speed being the constant of proportionality. The circumference of a circle is directly proportional to its diameter, with the constant of proportionality equal to π.
What is oral LD50?
Acute Oral Toxicity Tests The median lethal dose (or LD50) is defined as the dose of a test substance that is lethal for 50% of the animals in a dose group. LD50 values have been used to compare relative acute hazards of industrial chemicals, especially when no other toxicology data are available for the chemicals.
What is TCID50 assay?
The TCID50 (Median Tissue Culture Infectious Dose) assay is one method used to verify the viral titer of a testing virus. Host tissue cells are cultured on a well plate titer, and then varying dilutions of the testing viral fluid are added to the wells.
How do you calculate PFU from TCID50?
The titer as measured by TCID50 is 0.7 Log higher than the titer by standard plaque assay. To transform TCID50/ml into PFU/ml: T = 1 X 108.
What does TCID50 mean?
The TCID50 (Median Tissue Culture Infectious Dose) assay is one method used to verify the viral titer of a testing virus.
What is dilution factor in chemistry?
Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the volume of the final (dilute) solution1, that is, the ratio of V1 to V2. or. V1 : V2.
What TCID 50?
TCID50 is a common assay type The number of infectious virus particles is frequently quantified by using the Median Tissue Culture Infectious Dose (TCID50) assay. The assay works by adding a serial dilution of the virus sample to cells in a 96 well plate format.
Why is TCID50 important?
What is the LD50 of Tylenol?
The LD50 for acetaminophen (Tylenol)= 2402 mg/kg (rat, oral).
Which is an example of the Reed Muench method?
The Reed–Muench method is a simple method for determining 50% endpoints in experimental biology, that is, the concentration of a test substance that produces an effect of interest in half of the test units. Examples include LD50 (the median lethal dose of a toxin or pathogen), EC50 and IC50…
What is the Reed and Muench end point dilution formula?
Reed and Muench method log1050% end point dilution = log10of dilution showing a mortality next above 50% – (difference of logarithms × logarithm of dilution factor).
How did Reed and Muench calculate the median lethal dose?
Reed and Muench, 1938, have designed a well known method to calculate the median lethal dose (LD50) based on endpoint dilution assay outputs http://aje.oxfordjournals.org/content/27/3/493.full.pdf+html.