What is P1 gene?

What is P1 gene?

P1 genes expressed in the lytic pathway are those involved in the timing of phage development, in replication (from a “lytic” origin different from oriR), in the formation of phage particles, including headful packaging, and finally in cell lysis to release the phage progeny.

Is P1 a temperate phage?

Phage P1 is a temperate phage of the Myoviridae family, possessing an icosahedral head and a long, contractile tail. The phage devotes several genes to controlling replication and partitioning of the plasmid so that it is stably maintained at low copy number.

What is T1 phage?

Bacteriophage T1 is another member of the classic seven T phage. It is also an obligately lytic virus of E. coli, but unlike T4 it is a member of the Siphoviridae family, having an icosahedral head and a long, flexible, noncontractile tail. The T1 genome consists of a single dsDNA molecule of just under 50 000 bp.

What does a temperate phage do?

Temperate phages are considered as natural vectors for gene transmission among bacteria due to the ability to integrate their genomes into a host chromosome, therefore, affect the fitness and phenotype of host bacteria.

Are viruses host specific?

Viruses are host-specific because they only can attach to and infect cells of certain organisms. Cells that a virus may use to replicate are called permissive.

What are bacteriophage vectors name the two phage vectors that are commonly used?

Two types of vectors are most commonly used: E. coli plasmid vectors and bacteriophage λ vectors. Plasmid vectors replicate along with their host cells, while λ vectors replicate as lytic viruses, killing the host cell and packaging the DNA into virions (Chapter 6).

Which phage is known as temperate phage?

P1 is a temperate bacteriophage (phage) that infects Escherichia coli and some other bacteria. When undergoing a lysogenic cycle, the phage genome exists as a plasmid in the bacterium, unlike other phages (e.g., the lambda phage) that integrate into the host DNA.

What is a temperate phage quizlet?

a host cell that carries a prophage. temperate phage. – bacteriophage that have the option of entering the lysogenic cycle or the lytic cycle. – doesn’t form clear plaques the way lytic phages do because only a fraction of the host population is lysed. – slight cloudiness in plaque.

What is T1 virus?

Tunavirus (synonyms T1-like phages, T1-like viruses, Tunalikevirus) is a genus of viruses in the order Caudovirales, in the family Drexlerviridae. Bacteria serve as natural hosts. There are currently 14 species in this genus, including the type species Escherichia virus T1.

What does T stand for in T4 bacteriophage?

Figure 2. The structure of the bacteriophage T4 head. The symmetry of the gp23* major capsid protein shell is characterized by triangulation numbers Tend = 13 laevo and Tmid = 20 (h1 = 3, k1 = 1, h2 = 4 and k2 = 2) [26,27].

Which phase is a temperate phage?

A bacteriophage that displays lysogenic life cycle in contrast to virulent phage that does not have the ability to display lysogeny (especially following mutation). Many temperate phages are capable of integrating their genome into the genome of their host.

What determines viral specificity?

For most viruses, the molecular basis for this specificity is that a particular surface molecule known as the viral receptor must be found on the host cell surface for the virus to attach.