Table of Contents
- 1 What is the principle of the Gram stain process quizlet?
- 2 What is the principle of Gram staining How does it differentiates between bacteria?
- 3 What is the importance of Gram staining?
- 4 What is the purpose of the Gram stain quizlet?
- 5 What is the current theory about the mechanism of the Gram stain reaction?
- 6 What is the factor behind an organism being Gram (+) or Gram?
- 7 What are the four steps of Gram staining?
- 8 What is the most important step in Gram staining?
- 9 What are some advantages and disadvantages of Gram staining?
What is the principle of the Gram stain process quizlet?
Principle: Gram stain is differential stain which a decolorization step occurs between the application of 2 basic stains.
What is the principle of Gram staining How does it differentiates between bacteria?
The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.
What is the principle purpose of a simple stain?
The simple stain can be used to determine cell shape, size, and arrangement. True to its name, the simple stain is a very simple staining procedure involving only one stain. You may choose from methylene blue, Gram safranin, and Gram crystal violet.
What is the importance of Gram staining?
The main benefit of a gram stain is that it helps your doctor learn if you have a bacterial infection, and it determines what type of bacteria are causing it. This can help your doctor determine an effective treatment plan.
What is the purpose of the Gram stain quizlet?
What is the purpose of Gram Stain? To be able to determine the composition of the cell wall. The advantage of this staining procedure is that those cells that decolorize can be differetiated from the cells that resist decolorization by alcohol.
What is the principle behind the production of two different stains using Gram’s staining method?
Principle of Gram Stain The two major groups of bacteria can be divided into gram-positive and gram-negative. The Gram stain technique is based on the differential structure of the cellular membranes and cell walls of the two groups.
What is the current theory about the mechanism of the Gram stain reaction?
With the current theory behind Gram staining, it is thought that in Gram-positive bacteria, the crystal violet and iodine combine to form a larger molecule that precipitates out within the cell.
What is the factor behind an organism being Gram (+) or Gram?
In 1884, a bacteriologist named Christian Gram created a test that could determine if a bacterium had a thick, mesh-like membrane called peptidoglycan. Bacteria with thick peptidoglycan are called gram positive. If the peptidoglycan layer is thin, it’s classified as gram negative.
What does Gram stain indicate?
A Gram stain is a laboratory procedure used to detect the presence of bacteria and sometimes fungi in a sample taken from the site of a suspected infection. It gives relatively quick results as to whether bacteria or fungi are present and, if so, the general type(s).
What are the four steps of Gram staining?
Application of the primary stain Crystal Violet (CV) to a heat-fixed smear of bacterial culture. CV dissociates in aqueous solutions into CV+and Cl – ions.
What is the most important step in Gram staining?
Gram Staining : Place the slides on the staining rods. Cover the smear with crystal violet stain and leave for 1 minute. Wash carefully under running tap water. Flood the smear with Gram’s iodine solution and leave for 1 minute. Drain off the iodine Wash the slide for the again in a gentle stream of tap water.
What is the purpose of the primary stain in Gram staining?
The procedure is based on the reaction between peptidoglycan in the cell walls of some bacteria. The Gram stain involves staining bacteria, fixing the color with a mordant, decolorizing the cells, and applying a counterstain. The primary stain (crystal violet) binds to peptidoglycan, coloring cells purple.
What are some advantages and disadvantages of Gram staining?
Here are the advantages and disadvantages of Gram staining: 1. It gives quick results when examining infections . In most cases, Gram stains are performed on biopsy or bodily fluids when infection is suspected, and they yield results much more quickly than other methods, such as culturing.