What is the purpose of blood in the blood agar medium quizlet?

What is the purpose of blood in the blood agar medium quizlet?

Explain the purpose of the following: Blood in the blood agar medium. Blood acts as an enrichment ingredient for the cultivation of fastidious organisms. Blood also permits demonstrations of hemolytic properties. You just studied 8 terms!

What is purpose of blood in sheep blood agar plate?

Blood agar contains general nutrients and 5% sheep blood. It is useful for cultivating fastidious organisms and for determining the hemolytic capabilities of an organism. Some bacteria produce exoenzymes that lyse red blood cells and degrade hemoglobin; these are called hemolysins.

What does blood agar reveal?

Blood Agar is used to grow a wide range of pathogens particularly those that are more difficult to grow such as Haemophilus influenzae, Streptococcus pneumoniae and Neisseria species. It is also required to detect and differentiate haemolytic bacteria, especially Streptococcus species.

What is purpose of blood in sheep blood agar plate quizlet?

Blood agar plate- tripticase soy agar plate containing 5% sheep red blood cells which supports the growth of most medically important bacteria and it is used to detect the hemolysis patterns of streptococci isolated from clinical specimens.

How is blood agar a differential medium quizlet?

Blood agar is Differential and enrich. Beta-hemolysis is complete hemolysis. It is characterized by a clear (transparent) zone surrounding the colonies. An indistinct zone of partial destruction of red blood cells (RBCs) appears around the colony, often accompanied by a greenish to brownish discoloration of the medium.

What is the purpose of the horse blood in the blood agar when identifying haemolytic bacteria?

Horse blood allows detection of hemolytic reactions and supplies both the X factor (heme) and the V factor (nicotinamide adenine dinucleotide, NAD), necessary for the growth of many bacterial species, including Haemophilus influenzae, which requires both the X and V factors.

What is the purpose of Columbia CNA with 5% sheep blood agar?

BD Columbia CNA Agar with 5% Sheep Blood is a selective medium used for the isolation of gram-positive bacteria (especially staphylococci and streptococci) from clinical specimens. *Adjusted and/or supplemented as required to meet performance criteria.

What are the nutrients in blood agar?

Although similar to the idea of nutrient agar media, Blood agar media contains five to ten percent sheep or rabbit blood. Blood agar composition consists of Beef extract (nitrogen source), Blood (nitrogen, amino acids, and carbon source), Sodium chloride (maintains osmotic balance), agar (solidifying agent).

Is blood agar an enriched medium or a differential medium quizlet?

Blood Agar Plates (BAP) is Differential, selctive or enriched? Blood agar is Differential and enrich. Beta-hemolysis is complete hemolysis. It is characterized by a clear (transparent) zone surrounding the colonies.

Is blood agar a differential and or a selective medium explain?

A selective media is composed of specific ingredients to inhibit the growth of certain species of microbes in a mixed culture while allowing others to grow. Blood agar is a differential medium that distinguishes bacterial species by their ability to break down red blood cells.

Why is blood agar useful as a primary isolation medium?

Blood Agar is used as a primary isolation medium for most clinical samples. It is a rich medium that can support the growth of many pathogens. Because it contains red blood cells, it is differential showing which colonies can cause hemolysis.

Is blood agar an all purpose media?

Blood Agar (BA) this all-purpose enriched medium consists of tryptone Soy Agar with 5% sheep blood which aids the growth of many pathogenic microbes. The blood also provides the medium with differential properties because certain pathogens can hemolysis or break down the blood cells to access the iron they contain.

Why is sheep blood used in making blood agar?

Sheep blood has been preffered source in the Blood agar due to the fact that sheep RBCs are most sensitive to the the hemolytic toxins released by bacterial cells thus causing hemolytic zones around the colonies over the period of time.

What is the procedure for blood agar?

Procedure for the preparation of Blood Agar Prepare the blood agar base as instructed by the manufacturer. Sterilize by autoclaving at 121°C for 15 minutes. Transfer thus prepared blood agar base to a 50°C water bath. When the agar base is cooled to 50°C, add sterile blood aseptically and mix well gently. Avoid the formation of air bubbles.