Table of Contents
What part of the microscope uses high power?
Condenser Lens: The purpose of the condenser lens is to focus the light onto the specimen. Condenser lenses are most useful at the highest powers (400x and above).
What part of the microscope brings an object into focus at high power?
Coarse Adjustment Knob
Coarse Adjustment Knob- The coarse adjustment knob located on the arm of the microscope moves the stage up and down to bring the specimen into focus.
How would you bring an object to focus under the microscope?
- Start by rotating the objective lens to lowest power.
- Place a slide on the stage, label side up, with the coverslip centered.
- On LOW POWER ONLY, use the coarse focus knob to get the object into focus.
- If you cannot see anything, move the slide slightly while viewing and focusing.
How do you use a high powered microscope?
When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective. Re-focus on the image and then switch to the next highest power.
How do you focus a microscope on a higher power objective?
If you want to go to a higher power objective, do so, then focus again with the fine focus knob only. If you use the coarse adjustment knob under a higher power objective the stage may raise or lower too rapidly for you to ever bring the microscope into focus and resolve the image.
How many eyepieces are in a high power microscope?
High power microscopes usually have 10x eyepieces lenses, so there is only one column above the 4x objective. If you wanted to look at an amoeba that is 150µm long (0.15mm) then you would use either the 40x or 100x objectives.
How to demonstrate depth of focus in Higer power?
A common exercise to demonstrate depth of focus involves laying three different colored threads one on top of the other. As the observer focuses down, first the top thread comes into focus, then the middle one, and finally the bottom one. On higer power objectives one may go out of focus as another comes into focus.
What to do if you can’t see anything in a microscope?
If you cannot see anything, move the slide slightly while viewing and focusing. If nothing appears, reduce the light and repeat step 4. Once in focus on low power, center the object of interest by moving the slide. Rotate the objective to the medium power and adjust the fine focus only.