Table of Contents
- 1 Why is it important to center a specimen on low power?
- 2 Why must the specimen be centered before switching to high power?
- 3 What happens when you move the iris diaphragm lever?
- 4 What is the movement of the slide as you move the specimen from left to right?
- 5 How do you focus on a specimen in a microscope?
- 6 What happens if you grab the wrong knob on a microscope?
Why is it important to center a specimen on low power?
The specimen must be centered in the field of view on low power before going to high power because on high power, it is difficult to find the specimen. Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope.
Why are specimens upside down?
Microscopes invert images which makes the picture appear to be upside down. The reason this happens is that microscopes use two lenses to help magnify the image. Some microscopes have additional magnification settings which will turn the image right-side-up.
Why must the specimen be centered before switching to high power?
You must have the object centered before you change objectives to increase the magnification, because the field of view becomes smaller; if the object is off to the side, it may disappear when you go to higher magnification. For best viewing at high power, white light is essential.
What should you move down so that the objective lens will be near to the specimen?
THE OBJECTIVE SHOULD BE POSITIONED ABOUT 1/4″ – 3/8″ ABOVE THE SLIDE. 4. PLACE THE SLIDE ON THE MICROSCOPE STAGE SUCH THAT THE PORTION OF THE SLIDE YOU WANT TO VIEW IS UNDER THE OBJECTIVE. YOU MAY HAVE TO MOVE THE SLIDE AROUND ON THE STAGE OF THE MICROSCOPE TO BRING THE SPECIMEN INTO THE VIEWING AREA.
What happens when you move the iris diaphragm lever?
iris diaphragm lever: moving the lever to the left increases the light; moving the lever to the right decreases the light.
What happens when you switch from low to high power on a microscope?
When you switch to a higher power, the field of view is closes in. You will see more of an object on low power. The depth of focus is greatest on the lowest power objective. Each time you switch to a higher power, the depth of focus is reduced.
What is the movement of the slide as you move the specimen from left to right?
When you view a specimen through a microscope, you are viewing an image through multiple lenses. As a result, the image is upside down and back-to-front so when you move the slide to the right, the image moves to the left and vice versa!
What happens when you move the slide on a microscope to the right?
A specimen that is right-side up and facing right on the microscope slide will appear upside-down and facing left when viewed through a microscope, and vice versa. Similarly, if the slide is moved left while looking through the microscope, it will appear to move right, and if moved down, it will seem to move up.
How do you focus on a specimen in a microscope?
FOCUS ON SPECIMEN, FIRST USING THE COARSE AND THEN THE FINE FOCUS CONTROLS. YOU MAY HAVE TO MOVE THE SLIDE AROUND ON THE STAGE OF THE MICROSCOPE TO BRING THE SPECIMEN INTO THE VIEWING AREA. ADJUST THE POSITION OF THE OCULARS (the interocular distance) SO THAT A SINGLE IMAGE CAN BE SEEN WHEN LOOKING THROUGH BOTH OCULARS AT THE SAME TIME.
Why does the image move when you move the slide?
When you move the slide to the left, you will see the image go right when looking in the eyepiece. This is because everything is backwards in the microscope image. How does an image of an object seen under a microscope appear to move when you move the object?
What happens if you grab the wrong knob on a microscope?
When viewing a specimen, your eyes will be at the eyepieces, and if you grab the wrong knob by accident, you can lose your image at best, and damage the microscope at worst. Don’t assume you remember where the key knobs are.