Table of Contents
- 1 Why is it important to use a new tip when putting solutions into each of your sample tubes?
- 2 Why do we need to touch the end of the pipette tip into the container when dispensing a solution or sample?
- 3 Why do you have to carefully select the pipette that you use in your work?
- 4 Why is it important to change the pipette tip quizlet?
- 5 When filling up the pipette the tip of the pipette must be submerged in the solution to be filled?
- 6 Why is it important to wipe the sides of the pipette prior to dispensing?
- 7 Why are only sterile pipette tips used in the lab quizlet?
- 8 Do you need to remove the last drop of the solution in the pipette?
- 9 How do you pipe a solution into a Pipet?
- 10 What happens if you aspirate a pipette too shallow?
Why is it important to use a new tip when putting solutions into each of your sample tubes?
To avoid carry-over contamination: Use filter tips or positive displacement tips to prevent aerosol transfer from the sample into the pipette body, and again to the next sample.
Why do we need to touch the end of the pipette tip into the container when dispensing a solution or sample?
When using forward mode pipetting, dispense the sample while lightly touching the pipette tip against the side of the receiving container, so that the entire sample can be dispensed out of the pipette tip and no droplets remain sticking to the tip.
Why is it important to attach a new pipette tip before each use of the micropipette?
To prepare, fill the glass beaker with tap water. It is good etiquette to set the pipette to the correct volume before attaching a pipette tip. This is in order to reduce the risk of contaming the tip once it is attached to the pipette.
Why do you have to carefully select the pipette that you use in your work?
The specific lab application can help to steer tip type, whether it’s sterile, filtered, low-retention, wide bore, or more. It’s important to remember that the pipette and tip work together as a system. The liquid being pipetted is also an important consideration in tip selection.
Why is it important to change the pipette tip quizlet?
It is important to change pipette tips out after every use to prevent the cross contamination between liquid substances.
Why is keeping the vertical position of the pipette in dispensing the solution important?
The pipette should be held in a vertical position (tilting it may cause a change in sample volume) and the tip should not be allowed to touch the sides or bottom of the sample vessel.
When filling up the pipette the tip of the pipette must be submerged in the solution to be filled?
Immerse the tip to the proper depth during aspiration Before aspirating, immerse the tip adequately below the meniscus. Large volume pipettes (1-5 mL) should be immersed to 5-6 mm, while smaller volume pipettes should be immersed to 2-3 mm.
Why is it important to wipe the sides of the pipette prior to dispensing?
Wipe the tip against the edge of the vessel to remove excess liquid outside the tip before dispensing. In any case pipetting will not be accurate; some liquid will stay inside the tip. Aspiration and dispensing should be done slowly.
When filling a liquid solution into the tip you must depress the plunger to the?
6. Insert the tip of the pipette into to the tube or container where the liquid will be deposited. 7. Depress the plunger fully to the second stop point to deposit the liquid into the desired tube.
Why are only sterile pipette tips used in the lab quizlet?
Why are only sterile pipette tips used in the lab? To avoid contamination of the sample. You just studied 4 terms!
Do you need to remove the last drop of the solution in the pipette?
As you should (hopefully) know, when you are using a volumetric pipette, you should let the liquid drain out of the bottom and there will be a little left in the tip because of the surface tension. But you should NOT squeeze out that last drop no matter how tempting it may be!
Where do you put the tip of a Pipet?
Insert the tip of the pipet into the beaker of solution so that it is about 1/4” from the bottom. Be sure not to press the tip against the bottom of the container.
How do you pipe a solution into a Pipet?
Draw a small amount of the solution you wish to pipet into the pipet. Tilt and roll the pipet so that the solution has contact with all the inside surfaces. Discard the solution from the rinses. Repeat this rinse at least once more.
What happens if you aspirate a pipette too shallow?
The problems that occur when a pipette tip is immersed too shallowly are pretty easy to understand—if you don’t immerse your tip deeply enough, when you aspirate you run the risk of the tip ending up above the liquid before the plunger reaches the top of the stroke. The end result is air in the pipette tip and too little volume transferred.
How much immersion is needed in a pipette?
Studies show that improper immersion can alter the delivered volume by 2.2%. 2 To get optimal accuracy and precision out of your pipette, remember to pay attention to how deep your pipette tip is immersed into your sample— 2–3 mm for pipette volumes of 1–100 μL, 2–4 mm for 101–1000 μL, and 3–6 mm for volumes larger than 1 mL.