Table of Contents
- 1 Why might it be important to complete morphological and culture characteristics before beginning physiological tests?
- 2 How are the end products of biochemical reactions detected in culture media?
- 3 Why is it important to inoculate only a single tube per organism when performing the MRVP tests?
- 4 What is the purpose of biochemical tests?
- 5 Why is it necessary to determine cultural characteristics of microorganisms?
Why might it be important to complete morphological and culture characteristics before beginning physiological tests?
Terms in this set (46) Why is it important to complete morphological and cultural characterizations before pursuing physiological testing? Physiology is a display of what proteins and enzymes they are producing which comes from their genes.
Which of the following tests produce acid end products?
Lab 12 Physiological Characteristics of Bacteria
| Question | Answer |
|---|---|
| How are acids or alkalies detected in culture mediums? | By the use of pH indicators |
| Name two tests for reactions that produce acid end products; | Sugar fermentation and fat hydrolysis |
How are the end products of biochemical reactions detected in culture media?
LR36-38: End products of biochemical reactions are often acids or alkalies. How are these products typically detected in a culture medium? pH indicators (such as phenol red) are used to dye the medium, which will change color in response to pH changes. LR36-38: End products of biochemical reactions are sometimes gases.
Why is it important to complete morphological and cultural characteristics?
Why is it important to complete morphological and cultural characterizations before pursuing physiological testing? Morphological and cultural characterization are necessary to help with the preliminary classification. It helps point in the right direction of which tests are appropriate for further identification.
Why is it important to inoculate only a single tube per organism when performing the MRVP tests?
Why is it important to inoculate only a single tube per organism when performing the MRVP tests? A single tube is used to ensure that incubation conditions for both the MR and VP test are the same. During lab, your lab partner bumps into you, jostling your arm while you are inoculating your SIM tube.
Why is it important to use pure cultures when performing fermentation tests on bacteria?
The importance of having a pure culture, and not a mixed culture, when performing biochemical testing is that a pure culture may react much differently in isolation than when it is combined with other species. A mixed species entails contamination and, as such, the control of the experiment is lost.
What is the purpose of biochemical tests?
Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities of different bacteria.
What is the purpose of using biochemical tests when identifying an unknown organism?
Microbial biochemistry tests shorten the time required to identify microbes, reduce costs, and ensure or enhance the accuracy of identification of an unknown sample. It is the fastest developing trend in microbial identification.
Why is it necessary to determine cultural characteristics of microorganisms?
Study of cultural characteristics of microorganism is done with a purpose to distinguish different microorganisms into various taxonomic groups. So the objective of this experiment is to understand different characteristics of microorganisms and to classify them into various groups basing on their characteristics.
Why is it important to observe and describe the morphology of a colony?
Colony morphology is a method that scientists use to describe the characteristics of an individual colony of bacteria growing on agar in a Petri dish. It can be used to help to identify them. Colonies differ in their shape, size, colour and texture.