Table of Contents
What happens to the field of view as you go from low to high power?
The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power.
What happens to the image of the E as you go from scanning to low power to high power?
How will the image of the letter E change when you switch from low power to high power? The image will be larger, and less of the letter will be visable when I look through the eyepiece.
Why do you need to be extremely careful when you are using the high power lens?
Objective Lenses – The objective lens gathers light from the specimen, magnifies the image of the specimen, and projects the magnified image into the body tube. You must be very careful when using the high power lenses so you do not jam them into the slide.
What happens to the field of view when you increase magnification?
In short, as magnification increases, the field of view decreases. When looking through a high power compound microscope it can be difficult to determine what you will see through the eyepieces at different magnifications.
What do you think would happen if you tried to view a slide using the oil immersion lens but forgot to add the oil?
What would you observe if you forgot to use oil with the oil-immersion lens? The purpose of the oil is to contain the light rays and direct them upwards. If oil was not used, the rays would scatter, and the image would also be less magnified and lower clarity.
What is the effect of shifting from LPO to HPO on the microscope field?
Changing from low power to high power increases the magnification of a specimen. The amount an image is magnified is equal to the magnification of the ocular lens, or eyepiece, multiplied by the magnification of the objective lens.
What happened to the letter E when you move the slide to the left?
The “e” is flipped and backwards. 3. While looking through the microscope, move the slide to the left, notice which way the letter “e” moved. Now move the slide to the right.
What are the two functions of the eyepiece?
It is so named because it is usually the lens that is closest to the eye when someone looks through the device. The objective lens or mirror collects light and brings it to focus creating an image. The eyepiece is placed near the focal point of the objective to magnify this image.
What happens when you go from low power to high power on a microscope?
When you change from low power to high power on a microscope, the high-power objective lens moves directly over the specimen, and the low-power objective lens rotates away from the specimen. This change alters the magnification of a specimen, the light intensity, area of the field of view, depth of field, working distance and resolution.
What happens when you go from low power to high power on a camera?
Change in Magnification. Changing from low power to high power increases the magnification of a specimen. The amount an image is magnified is equal to the magnification of the ocular lens, or eyepiece, multiplied by the magnification of the objective lens. Usually, the ocular lens has a magnification of 10x.
Why is the microscope not in focus for my Left Eye?
IT IS LIKELY THAT WHILE THE IMAGE YOU ARE VIEWING MAY BE IN FOCUS FOR ONE OF YOUR EYES, IT IS NOT IN FOCUS FOR THE OTHER. ONE OF THE OCULARS ON YOUR MICROSCOPE HAS AN ADJUSTABLE FOCUS TO ACCOUNT FOR THIS. LOOK THROUGH THE NON-ADJUSTABLE RIGHT OCULAR AND COVER YOUR LEFT EYE WITH THE INDEX CARD THAT IS IN THE TOP DRAWER AT YOUR SEAT.
What happens to the amount of light transmitted to the eye?
The amount of light transmitted to your eye is greatest at the low power. When you switch to a higher power, light (and therefore resolving power, or the ability to distinguish two nearby objects as separate) is reduced. Compensate with the light control (sometimes called the iris diaphragm ).