Table of Contents
- 1 Would you expect the Rf number of a pigment to change if you altered the composition of the solvent?
- 2 What factors affect the Rf values of the different solutes used?
- 3 What would the Rf value of a pigment do if a different solvent was used?
- 4 Would you expect the Rf value of a pigment?
- 5 What is the importance of Rf value in chromatography?
- 6 What does the Rf value tell you in chromatography?
- 7 Does temperature affect chromatography?
- 8 Would you expect similar Rf values if you used a different solvent Why or why not?
- 9 How is RF calculated in a Chromatography lab?
- 10 What does the Rf value tell you in TLC?
- 11 What is the Rf value of a compound?
Would you expect the Rf number of a pigment to change if you altered the composition of the solvent?
Would you expect the Rf value of a pigment to change if we altered the composition of the solvent? If altering the composition of the solvent resulted in a change the solubility of the pigment in the solvent, the Rf value would also change.
What factors affect the Rf values of the different solutes used?
Rf values and reproducibility can be affected by a number of different factors such as layer thickness, moisture on the TLC plate, vessel saturation, temperature, depth of mobile phase, nature of the TLC plate, sample size, and solvent parameters. These effects normally cause an increase in Rf values.
How does temperature affect Rf value?
The temperature of the solvent and plate may make slight changes, since, for example, the solvent can often better dissolve the chemicals it is transporting at higher temperatures. The technique of the technician in applying the sample to the plate may also change the retention factor.
What would the Rf value of a pigment do if a different solvent was used?
The Rf value of a pigment would not be the same if a different solvent were used because each solvent is different. The solubility and size of the specific solvent will produce different results (No author mentioned.
Would you expect the Rf value of a pigment?
Solubility, the size of the particles and the pigments attractiveness to the paper based upon the pigment’s chemical structure. Would you expect the Rf value of the pigment to be the same if a different solvent was used? No, different solubilities would change the Rf value.
Would you expect similar Rf values if you used a different solvent?
If two substance have the same Rf value, they are likely but not necessarily the same compound. If they have different Rf, they are definitely different compounds.
What is the importance of Rf value in chromatography?
In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify compounds. The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin).
What does the Rf value tell you in chromatography?
The Rf values indicate how soluble the particular pigment is in the solvent by how high the pigment moves on the paper. Two pigments with the same Rf value are likely to be identical molecules. Small Rf values tend to indicate larger, less soluble pigments while the highly soluble pigments have an Rf value near to one.
What is the Rf value in chromatography?
Does temperature affect chromatography?
Temperature can affect the separation of components in all chromatography types. If the temperature rises, the heat transfers further energy to the solvent-giving the molecule the power to escape from the surface of the liquid hence increases the transfer of liquid to the vapor phase.
Would you expect similar Rf values if you used a different solvent Why or why not?
What caused the pigments to separate on the chromatography paper?
The process of chromatography separates molecules because of the different solubilities of the molecules in a selected solvent. The solvent carries the dissolved pigments as it moves up the paper. The pigments are carried at different rates because they are not equally soluble.
How is RF calculated in a Chromatography lab?
Rf is the relationship of the distance moved by a pigment to the distance moved by the solvent. It can be calculated by dividing the distance pigment migrated by distance solvent front migrated. Chromatography is used to separate mixtures of substances into their components.
What does the Rf value tell you in TLC?
The Rf value is defined as the ratio of the distance moved by the solute (i.e. the dye or pigment under test) and the distance moved by the the solvent (known as the Solvent front) along the paper, where both distances are measured from the common Origin or Application Baseline, that is the point where the sample is
How is the RF of a pigment calculated?
Rf is the relationship of the distance moved by a pigment to the distance moved by the solvent. It can be calculated by dividing the distance pigment migrated by distance solvent front migrated.
What is the Rf value of a compound?
The Rf value The retention factor, or Rf, is defined as the distance traveled by the compound divided by the distance traveled by the solvent. For example, if a compound travels 2.1 cm and the solvent front travels 2.8 cm, the Rf is.